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shRNA-mediated knockdown of <t>DDR2.</t> ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .
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shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .

Journal: Life

Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

doi: 10.3390/life14101260

Figure Lengend Snippet: shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .

Article Snippet: Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

Techniques: shRNA, Knockdown, Expressing, Western Blot

DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.

Journal: Life

Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

doi: 10.3390/life14101260

Figure Lengend Snippet: DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.

Article Snippet: Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

Techniques: Microscopy